| Accession | CRX056460 |
| Organism | root metagenome |
| Title | 16S amplicon sequencing of rice root microbiome sample 2OsRE2-2 |
| BioProject | PRJCA001629 |
| BioSample | SAMC088228 |
| Platform | Illumina MiSeq |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
DNA for each sample was extracted,then V5-V7(799F-1193R) of 16S rDNA was amplified by two-step PCR,and the amplicon was digested using Cas9/mt-gRNA1171,and second step PCR is 15 cycles.Finally,the amplicons were sequenced on Illumina HiSeq 2500 platform in pair-end 250 bp mode |
AMPLICON |
METAGENOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
Insert size (bp): 394
|
| Release date | 2020-01-04 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR062535 |
CRR062535_f1.fastq.gz
CRR062535_r2.fastq.gz
|
31.06
36.47
|
|
| Submitter | Lu yang Song (lyang.s@webmail.hzau.edu.cn) |
|---|
| Organization | National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University |
|---|
| Date submitted | 2019-08-04 |
|---|
