Accession | CRX056479 |
Organism | soil metagenome |
Title | 16S amplicon sequencing of bulk soil microbiome sample Bs1048-6 |
BioProject | PRJCA001629 |
BioSample | SAMC088247 |
Platform | Illumina HiSeq 2500 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
DNA for each sample was extracted,then V5-V7(799F-1193R) of 16S rDNA was not amplified by two-step PCR,and the amplicon was not digested using Cas9/mt-gRNA1048.Finally,the amplicons were sequenced on Illumina HiSeq 2500 platform in pair-end 250 bp mode |
AMPLICON |
METAGENOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
Insert size (bp): 394
|
Release date | 2020-01-04 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR062554 |
CRR062554_f1.fq.gz
CRR062554_r2.fq.gz
|
8.65
9.51
|
|
Submitter | Lu yang Song (lyang.s@webmail.hzau.edu.cn) |
Organization | National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University |
Date submitted | 2019-08-04 |