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实验基本信息
实验编号 CRX056486
物种名称 soil metagenome
标题 16S amplicon sequencing of bulk soil microbiome sample Bs1196-1
项目编号 PRJCA001629
样本编号 SAMC088254
测序平台 Illumina HiSeq 2500
建库信息
文库名称 文库构建方法 建库策略 样品来源 实验选择 文库布局
DNA for each sample was extracted,then V5-V7(799F-1193R) of 16S rDNA was amplified by two-step PCR,and the amplicon was digested using Cas9/mt-gRNA1196.Finally,the amplicons were sequenced on Illumina HiSeq 2500 platform in pair-end 250 bp mode AMPLICON METAGENOMIC PCR PAIRED
处理信息 Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
Insert size (bp): 394
发布日期2020-01-04
测序反应
Run编号 Run序列文件信息
File nameFile size (MB)
CRR062561 CRR062561_f1.fq.gz
CRR062561_r2.fq.gz
94.37
104.81
提交者Lu yang Song (lyang.s@webmail.hzau.edu.cn)
所属单位National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University
提交日期2019-08-04