Accession | CRX081439 |
Organism | leaf metagenome |
Title | 16S amplicon sequencing of rice leaf microbiome sample V4leaf2 |
BioProject | PRJCA001629 |
BioSample | SAMC125244 |
Platform | Illumina HiSeq 2500 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
DNA for each sample was extracted,then V4(515F-806R) of 16S rDNA was amplified by two-step PCR,and the amplicon was digested using Cas9/mt-gRNA780 and cp-gRNA578.Finally,the amplicons were sequenced on Illumina HiSeq 2500 platform in pair-end 250 bp mode |
AMPLICON |
METAGENOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
Insert size (bp): 300
|
Release date | 2020-01-04 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR104399 |
CRR104399_f1.fastq.gz
CRR104399_r2.fastq.gz
|
25.85
22.94
|
|
Submitter | Lu yang Song (lyang.s@webmail.hzau.edu.cn) |
Organization | National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University |
Date submitted | 2019-12-25 |