| Accession | CRX081439 |
| Organism | leaf metagenome |
| Title | 16S amplicon sequencing of rice leaf microbiome sample V4leaf2 |
| BioProject | PRJCA001629 |
| BioSample | SAMC125244 |
| Platform | Illumina HiSeq 2500 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
DNA for each sample was extracted,then V4(515F-806R) of 16S rDNA was amplified by two-step PCR,and the amplicon was digested using Cas9/mt-gRNA780 and cp-gRNA578.Finally,the amplicons were sequenced on Illumina HiSeq 2500 platform in pair-end 250 bp mode |
AMPLICON |
METAGENOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
Insert size (bp): 300
|
| Release date | 2020-01-04 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR104399 |
CRR104399_f1.fastq.gz
CRR104399_r2.fastq.gz
|
25.85
22.94
|
|
| Submitter | Lu yang Song (lyang.s@webmail.hzau.edu.cn) |
|---|
| Organization | National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University |
|---|
| Date submitted | 2019-12-25 |
|---|
