| 实验编号 | CRX081446 |
| 物种名称 | soil metagenome |
| 标题 | 16S amplicon sequencing of bulk soil microbiome sample V4BS3 |
| 项目编号 | PRJCA001629 |
| 样本编号 | SAMC125251 |
| 测序平台 | Illumina HiSeq 2500 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
DNA for each sample was extracted,then V4(515F-806R) of 16S rDNA was amplified by two-step PCR,and the amplicon was digested using Cas9/mt-gRNA780 and cp-gRNA578.Finally,the amplicons were sequenced on Illumina HiSeq 2500 platform in pair-end 250 bp mode |
AMPLICON |
METAGENOMIC |
PCR |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
Insert size (bp): 300
|
| 发布日期 | 2020-01-04 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR104406 |
CRR104406_f1.fastq.gz
CRR104406_r2.fastq.gz
|
26.63
23.63
|
|
| 提交者 | Lu yang Song (lyang.s@webmail.hzau.edu.cn) |
|---|
| 所属单位 | National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University |
|---|
| 提交日期 | 2019-12-25 |
|---|
