| 实验编号 | CRX071269 |
| 物种名称 | Scylla paramamosain |
| 标题 | HiC sequencing of mud crab |
| 项目编号 | PRJCA001790 |
| 样本编号 | SAMC109789 |
| 测序平台 | Illumina HiSeq X Ten |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
Chromatin was extratcted from the muscle then digested for 16 h with 400 U HindIII restriction enzyme (NEB) at 37 °C. DNA ends were labeled with biotin and incubated at 37 °C for 45 min, and the enzyme was inactivated with 20% SDS solution. DNA ligation was performed by the addition of T4 DNA ligase (NEB) and incubation at 16°C for 4-6 h. After ligation, proteinase K was added to reverse cross-linking during incubation at 65 °C overnight. DNA fragments were purified and dissolved in 86μL of water. Unligated ends were then removed. Purified DNA was fragmented to a size of 300-500 bp, and DNA ends were then repaired. DNA fragments labeled by biotin were finally separated on Dynabeads M-280 Streptavidin (Life Technologies). Hi-C libraries were controlled for quality and sequenced on an Illumina Hiseq X Ten sequencer. |
Hi-C |
GENOMIC |
PCR |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 500
|
| 发布日期 | 2021-03-12 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR083774 |
CRR083774_f1.fq.gz
CRR083774_r2.fq.gz
|
5,831.5
6,940.27
|
| CRR083775 |
CRR083775_f1.fq.gz
CRR083775_r2.fq.gz
|
5,820.27
6,904.6
|
| CRR083776 |
CRR083776_f1.fq.gz
CRR083776_r2.fq.gz
|
13,334.74
14,249.78
|
| CRR083777 |
CRR083777_f1.fq.gz
CRR083777_r2.fq.gz
|
346.04
365.11
|
| CRR083778 |
CRR083778_f1.fq.gz
CRR083778_r2.fq.gz
|
3,840.23
4,037.09
|
|
| 提交者 | Ming Zhao (mingzhao1@hotmail.com) |
|---|
| 所属单位 | East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences |
|---|
| 提交日期 | 2019-10-24 |
|---|
