Experiment information
Accession CRX072170
Organism Homo sapiens
Title PAX-SH-108-pre-RNAseq
BioProject PRJCA001647
BioSample SAMC114126
Platform Illumina HiSeq 2500
Library name Construction protocol Strategy Source Selection Layout
Total RNA was isolated using RNeasy mini kit (Qiagen, Germany). Strand-specific libraries were prepared using the TruSeq® Stranded Total RNA Sample Preparation kit (Illumina, USA) following the manufacturer’s instructions. Ribosomal RNA was removed from total RNA using Ribo-Zero rRNA removal beads.Purified libraries were quantified by Qubit® 2.0 Fluorometer (Life Technologies, USA) and validated by Agilent 2100 bioanalyzer (Agilent Technologies, USA) to confirm the insert size and calculate the mole concentration. Cluster was generated by cBot with the library diluted to 10 pM and then were sequenced on the Illumina HiSeq 2500 (Illumina, USA). RNA-Seq TRANSCRIPTOMIC RANDOM PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 435
Release date2020-12-31
Run accession Release date Run data file information
File nameFile size (MB)
CRR084915 2020-12-31 PAX-SH-108-pre-RNAseq_1.fq.gz
SubmitterJin Gu (jgu@tsinghua.edu.cn)
OrganizationTsinghua University
Date submitted2019-11-14
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