Experiment information
Accession CRX072228
Organism Homo sapiens
Title PAX-SH-116-pre-miRNAseq
BioProject PRJCA001647
BioSample SAMC114124
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA was extracted using a mirVana miRNA Isolation Kit (Ambion). Small RNAs had linkers ligated to them and bar-coded cDNAs were prepared using a TruSeq Small RNA Sample Prep Kit (Illumina) following the manufacturer’s instructions. The amplified cDNA constructs were purified from agarose gel in preparation for sequencing analysis using the Illumina HiSeq 2500(Illumina). miRNA-Seq TRANSCRIPTOMIC other FRAGMENT
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 350
Planned read length (bp): 50
Release date2020-12-31
Run
Run accession Release date Run data file information
File nameFile size (MB)
CRR084973 2020-12-31 PAX-SH-116-pre-miRNAseq.fg.gz 299.67
SubmitterJin Gu (jgu@tsinghua.edu.cn)
OrganizationTsinghua University
Date submitted2019-11-14
Related experiments
Experiments(167)