| Accession | CRX072232 |
| Organism | Homo sapiens |
| Title | PAX-SH-138-pre-miRNAseq |
| BioProject | PRJCA001647 |
| BioSample | SAMC114120 |
| Platform | Illumina HiSeq 2500 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total RNA was extracted using a mirVana miRNA Isolation Kit (Ambion). Small RNAs had linkers ligated to them and bar-coded cDNAs were prepared using a TruSeq Small RNA Sample Prep Kit (Illumina) following the manufacturer’s instructions. The amplified cDNA constructs were purified from agarose gel in preparation for sequencing analysis using the Illumina HiSeq 2500(Illumina). |
miRNA-Seq |
TRANSCRIPTOMIC |
other |
SINGLE
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 350
Planned read length (bp): 50
|
| Release date | 2020-12-31 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR084977 |
CRR084977.gz
|
318.72
|
|
| Submitter | Jin Gu (jgu@tsinghua.edu.cn) |
|---|
| Organization | Tsinghua University |
|---|
| Submitted date | 2019-11-14 |
|---|
