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实验基本信息
实验编号 CRX072249
物种名称 Homo sapiens
标题 PAX-BJ-181-post-miRNAseq
项目编号 PRJCA001647
样本编号 SAMC114163
测序平台 Illumina HiSeq 2500
建库信息
文库名称 文库构建方法 建库策略 样品来源 实验选择 文库布局
Total RNA was extracted using a mirVana miRNA Isolation Kit (Ambion). Small RNAs had linkers ligated to them and bar-coded cDNAs were prepared using a TruSeq Small RNA Sample Prep Kit (Illumina) following the manufacturer’s instructions. The amplified cDNA constructs were purified from agarose gel in preparation for sequencing analysis using the Illumina HiSeq 2500(Illumina). miRNA-Seq TRANSCRIPTOMIC other SINGLE
处理信息 Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 350
Planned read length (bp): 50
发布日期2020-12-31
测序反应
Run编号 Run序列文件信息
File nameFile size (MB)
CRR084994 CRR084994.gz 241.59
提交者Jin Gu (jgu@tsinghua.edu.cn)
所属单位Tsinghua University
提交日期2019-11-14
关联实验
Experiments(167)