Experiment information
Accession CRX072316
Organism Homo sapiens
Title PAX-SH-138-post-WES
BioProject PRJCA001647
BioSample SAMC114148
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
Genomic DNA was extracted using DNeasy Tissue Kit (QIAGEN). Exome capture was performed using the SureSelect Human All Exon V5 (Agilent Technologies) according to the manufacturer’s instructions. The quantity of libraries were assessed by Qubit® 2.0 Fluoromete. The quality and size of libraries were measured by 2100 Bioanalyzer High Sensitivity DNA Assay according to the reagent kit guide. For Illumina sequencing, the qualified libraries were applied to 2×125 bp paired-end sequencing on Illumina HiSeq 2500 (Illumina). WES GENOMIC Hybrid Selection PAIRED
Processing Planned read length (bp) for mate 1: 125
Planned read length (bp) for mate 2: 125
Insert size (bp): 233
Planned read length (bp): 50
Release date2020-12-31
Run
Run accession Release date Run data file information
File nameFile size (MB)
CRR085061 2020-12-31 PAX-SH-138-post-WES_1.fastq.gz
PAX-SH-138-post-WES_2.fastq.gz
5,354.67
6,027.55
SubmitterJin Gu (jgu@tsinghua.edu.cn)
OrganizationTsinghua University
Date submitted2019-11-14
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