| 实验编号 | CRX093200 |
| 物种名称 | Gallus gallus |
| 标题 | D100 |
| 项目编号 | PRJCA002238 |
| 样本编号 | SAMC136270 |
| 测序平台 | Illumina HiSeq 4000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
The extracted primary muscle cells and fat cells were subjected to preliminary quality inspection, respectively. Cell concentration and viability were determined using a Countstar BioMed Professional Immune Cell Counter (Life Science, Shanghai, China). Two single-cell suspensions of muscles containing cells and adipocytes were mixed. The volumes of liquid with similar concentrations were mixed in a ratio of 1:3 for the liquid and upper-layer cells. The mixed cell suspension was then adjusted to the ideal concentration of 1000 /μL. The Chromium Single Cell Controller (10x Genomics, San Francisco, CA) was used to analyze 3,000 recovered cells. The RNA from the barcoded cells was subsequently reverse-transcribed, and sequencing libraries were constructed with reagents from a Chromium Single Cell 3’ v2 reagent kit (10× Genomics) in accordance with the instructions provided by the manufacturer. Sequencing was performed with Illumina HiSeq 4000 as instructed by the manufacturer (CapitalBio Technology, Beijing, China). The sequencing length consisted of two parts: (i) 26 bp Read1, including a 16 bp barcode and a 10 bp unique molecular identifier (UMI), and (ii) 98 bp Read2, which was the sample RNA sequence. |
RNA-Seq |
TRANSCRIPTOMIC |
RT-PCR |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 124
Planned read length (bp) for mate 2: 124
Insert size (bp): 200
|
| 发布日期 | 2020-09-30 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR117597 |
CRR117597_f1.fq.gz
CRR117597_r2.fq.gz
|
8,149.83
8,909.24
|
| CRR117598 |
CRR117598_f1.fq.gz
CRR117598_r2.fq.gz
|
9,131.19
10,075.26
|
| CRR117599 |
CRR117599_f1.fq.gz
CRR117599_r2.fq.gz
|
11,889.62
13,115.47
|
| CRR117600 |
CRR117600_f1.fq.gz
CRR117600_r2.fq.gz
|
6,198.07
6,798.34
|
|
| 提交者 | Siyuan Xing (tcsxingsy@126.com) |
|---|
| 所属单位 | Institute of Animal Sciences, Chinese Academy of Agricultural Sciences |
|---|
| 提交日期 | 2020-02-21 |
|---|
