| 实验编号 | CRX094187 |
| 物种名称 | Locusta migratoria |
| 标题 | IG8h-1 |
| 项目编号 | PRJCA002298 |
| 样本编号 | SAMC139016 |
| 测序平台 | Illumina HiSeq 3000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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The total RNA was extracted from the collected locust brains by the TRIzol reagent (Invitrogen) according to the manufacturer’s protocol. The quantity and purity of total RNA extracted as described above were determined with RIN value >7.0 by ND-1000 Nanodrop (Thermo Fisher, USA) and Agilent 2200 Tapestation (Agilent Technologies, USA). The RNA integrity was examined by agarose gel electrophoresis. Then ribosomal RNA was removed from total RNA using EpicentreRibo-Zero rRNA Removal Kit, (Illumina company, USA). The rRNA depleted RNA was put to perform randomly fragmented into short segments and instantly used as template to synthesis the first cDNA by random hexamers. Next, the second cDNA was synthesized and RNA was digested by RNase H. The double-stranded DNA then was purified and added with bases A and adaptor in the 3’-end. After selection by AMPureXP beads, the second strand cDNA containing U was degraded by enzyme USEB. Finally, the cDNA library for sequencing was constructed by PCR amplification. Then the quality of cDNA library was also inspected and the sequencing was performed on an Illumina Hiseq3000. |
ssRNA-Seq |
TRANSCRIPTOMIC |
RT-PCR |
PAIRED
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|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
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| 发布日期 | 2020-02-29 |
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| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR118906 |
CRR118906_f1.fastq.gz
CRR118906_r2.fastq.gz
|
3,223.02
3,679.71
|
|
| 提交者 | Pengcheng Yang (yangpc@biols.ac.cn) |
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| 所属单位 | Chinese Academy of Sciences |
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| 提交日期 | 2020-02-28 |
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