| 实验编号 | CRX094782 |
| 物种名称 | Bacteria |
| 标题 | 208 |
| 项目编号 | PRJCA002314 |
| 样本编号 | SAMC140781 |
| 测序平台 | Illumina HiSeq 2500 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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We used three gut microbiota DNA extraction strategies based on the insect body length. Large individuals (>5 mm) were extracted as one sample. Five intermediate size individuals (2-5 mm) were extracted as a sample. Ten small individuals (<5 mm) were extracted as a sample.DNA extraction were followed the Fast DNA Stool Mini Kit protocol (Qiagen).PCR were followed HiFi HotStart DNA Polymerase (Kapa Biosystems) protocol to get 16s rDNA of bacteria. |
AMPLICON |
METAGENOMIC |
PCR |
PAIRED
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|
| 处理信息 |
Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
Insert size (bp): 450
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| 发布日期 | 2020-03-04 |
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| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR119556 |
CRR119556_f1.fastq.gz
CRR119556_r2.fastq.gz
|
4.99
5.74
|
|
| 提交者 | qi wang (wangqi@wh.iov.cn) |
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| 所属单位 | anhui university |
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| 提交日期 | 2020-03-04 |
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