| 实验编号 | CRX107897 |
| 物种名称 | Homo sapiens |
| 标题 | P02_N |
| 项目编号 | PRJCA002596 |
| 样本编号 | SAMC181793 |
| 测序平台 | Illumina HiSeq 4000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
Fresh gastric tumor and adjacent tissues were cut into approximately 1 mm3 pieces in RPMI-1640 medium (Invitrogen) with 10% fetal bovine serum (FBS; ScienCell) and enzymatically digested with a MACS tumor dissociation kit (Miltenyi Biotec) for 30 min on a rotor at 37°C, according to the manufacturer’s instructions. After filtration with a 70 μm Cell-Strainer (BD) in RPMI-1640 medium (Invitrogen), the suspended cells were centrifuged at 400× g for 5 min. The single cell suspensions were stained with 7-AAD Viability Staining Solution (Cat# 00-6993-50, eBiocience) for flow cytometry (FACS), performed on a BD Aria III instrument. Based on FACS analysis, 1 × 105 living cells were sorted into 1.5 ml tubes with sorting buffer, and counted manually under the microscope. Then, single cells were processed with the GemCode Single Cell Platform using the 3’ GemCode Gel Bead, Chip and Library Kits (10x Genomics) as per the manufacturer’s protocol. |
RNA-Seq |
TRANSCRIPTOMIC |
other |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 350
|
| 发布日期 | 2020-05-02 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR133257 |
CRR133257_f1.fastq.gz
CRR133257_r2.fastq.gz
|
4,823.11
4,898.1
|
| CRR133258 |
CRR133258_f1.fastq.gz
CRR133258_r2.fastq.gz
|
4,784.93
4,882.2
|
| CRR133259 |
CRR133259_f1.fastq.gz
CRR133259_r2.fastq.gz
|
5,421.87
5,532.83
|
| CRR133260 |
CRR133260_f1.fastq.gz
CRR133260_r2.fastq.gz
|
8,038.5
8,228.41
|
|
| 提交者 | Ruichai Lin (linruichai@clabee.com) |
|---|
| 所属单位 | Clabee Genomics |
|---|
| 提交日期 | 2020-04-28 |
|---|
