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实验基本信息
实验编号 CRX107904
物种名称 Homo sapiens
标题 P05_T
项目编号 PRJCA002596
样本编号 SAMC181798
测序平台 Illumina HiSeq 4000
建库信息
文库名称 文库构建方法 建库策略 样品来源 实验选择 文库布局
Fresh gastric tumor and adjacent tissues were cut into approximately 1 mm3 pieces in RPMI-1640 medium (Invitrogen) with 10% fetal bovine serum (FBS; ScienCell) and enzymatically digested with a MACS tumor dissociation kit (Miltenyi Biotec) for 30 min on a rotor at 37°C, according to the manufacturer’s instructions. After filtration with a 70 μm Cell-Strainer (BD) in RPMI-1640 medium (Invitrogen), the suspended cells were centrifuged at 400× g for 5 min. The single cell suspensions were stained with 7-AAD Viability Staining Solution (Cat# 00-6993-50, eBiocience) for flow cytometry (FACS), performed on a BD Aria III instrument. Based on FACS analysis, 1 × 105 living cells were sorted into 1.5 ml tubes with sorting buffer, and counted manually under the microscope. Then, single cells were processed with the GemCode Single Cell Platform using the 3’ GemCode Gel Bead, Chip and Library Kits (10x Genomics) as per the manufacturer’s protocol. RNA-Seq TRANSCRIPTOMIC other PAIRED
处理信息 Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 350
发布日期2020-05-02
测序反应
Run编号 Run序列文件信息
File nameFile size (MB)
CRR133309 CRR133309_f1.fastq.gz
CRR133309_r2.fastq.gz
2,691.86
2,553.71
CRR133310 CRR133310_f1.fastq.gz
CRR133310_r2.fastq.gz
2,679.4
2,556.29
CRR133311 CRR133311_f1.fastq.gz
CRR133311_r2.fastq.gz
1,112.57
1,072.22
CRR133312 CRR133312_f1.fastq.gz
CRR133312_r2.fastq.gz
2,487.3
2,370.48
CRR133313 CRR133313_f1.fastq.gz
CRR133313_r2.fastq.gz
2,459.91
2,358.88
CRR133314 CRR133314_f1.fastq.gz
CRR133314_r2.fastq.gz
944.19
912.57
CRR133315 CRR133315_f1.fastq.gz
CRR133315_r2.fastq.gz
2,928.33
2,768.73
CRR133316 CRR133316_f1.fastq.gz
CRR133316_r2.fastq.gz
2,904.65
2,764.44
CRR133317 CRR133317_f1.fastq.gz
CRR133317_r2.fastq.gz
1,114.13
1,069.15
CRR133318 CRR133318_f1.fastq.gz
CRR133318_r2.fastq.gz
2,225.42
2,112.43
CRR133319 CRR133319_f1.fastq.gz
CRR133319_r2.fastq.gz
2,204.47
2,104.78
CRR133320 CRR133320_f1.fastq.gz
CRR133320_r2.fastq.gz
837.53
806.71
提交者Ruichai Lin (linruichai@clabee.com)
所属单位Clabee Genomics
提交日期2020-04-28