| 实验编号 | CRX107981 |
| 物种名称 | Dendrobium nobile |
| 标题 | Dendrobium nobile.-CK1 |
| 项目编号 | PRJCA002604 |
| 样本编号 | SAMC182244 |
| 测序平台 | Illumina HiSeq 4000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
A total of 1.5 mg of total RNA per biological replicate was prepared to construct the cDNA library according to the TruSeq RNA Sample Prep Kit (Illumina, USA). Briefly, poly-T oligo-attached magnetic beads (Illumina, USA) were used to isolate mRNA from total RNA. Then the mRNA was fragmented into ~200 bp length segments using a fragmentation buffer. After that, the first-strand cDNA was synthesized based on short fragmented mRNA. Subsequently, the cDNA fragments were end-paired, A-tailed, and added adaptor after the double-stranded cDNA synthesis. Finally the cDNA was amplified by PCR for 15 cycles. |
RNA-Seq |
TRANSCRIPTOMIC |
cDNA |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 200
|
| 发布日期 | 2020-04-29 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR133403 |
CRR133403_f1.fastq.gz
CRR133403_r2.fastq.gz
|
1,983.98
2,284
|
| CRR133404 |
CRR133404_f1.fastq.gz
CRR133404_r2.fastq.gz
|
1,926.29
2,205.24
|
| CRR133405 |
CRR133405_f1.fastq.gz
CRR133405_r2.fastq.gz
|
1,916.9
2,187.98
|
|
| 提交者 | Bin Zhu (zhugg130@126.com) |
|---|
| 所属单位 | Guizhou Normal University |
|---|
| 提交日期 | 2020-04-29 |
|---|
