Accession | CRX108435 |
Organism | Mus musculus |
Title | 2D Hi-C rep1 |
BioProject | PRJCA002611 |
BioSample | SAMC182402 |
Platform | Illumina HiSeq X Ten |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The Hi-C chimeric DNA were prepared by "in-situ Hi-C" method with minor modifications, Briefly, nuclei released from ten million crosslinked cells were digested with Mbo I (NEB). After biotin fill-in and ligation in nuclei, biotin labeled ligation product were fragmented with Cavrios M220, the molecular with size ranging from 300bp to 500bp were selected for the generation of library. The Hi-C library were prepared using NEBNext Ultra DNA Library Prep Kit for Illumina with modifications. Briefly, after end-repair and adaptor ligation with NEB modules, biotin labeled validated chimeric DNA were selected with Streptavidin C1 beads (Invitrogen). Hi-C library was amplified after DNA elution by inculating at 98° C. |
Hi-C |
GENOMIC |
other |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
|
Release date | 2020-05-02 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR133859 |
CRR133859_f1.fastq.gz
CRR133859_r2.fastq.gz
|
16,732.35
18,787.84
|
|
Submitter | HUA LI (kaikaixinxin@sjtu.edu.cn) |
Organization | Shanghai Jiao Tong University |
Date submitted | 2020-04-30 |