Experiment information
Accession CRX108438
Organism Mus musculus
Title 3D Hi-C rep2
BioProject PRJCA002611
BioSample SAMC182405
Platform Illumina HiSeq X Ten
Library name Construction protocol Strategy Source Selection Layout
The Hi-C chimeric DNA were prepared by "in-situ Hi-C" method with minor modifications, Briefly, nuclei released from ten million crosslinked cells were digested with Mbo I (NEB). After biotin fill-in and ligation in nuclei, biotin labeled ligation product were fragmented with Cavrios M220, the molecular with size ranging from 300bp to 500bp were selected for the generation of library. The Hi-C library were prepared using NEBNext Ultra DNA Library Prep Kit for Illumina with modifications. Briefly, after end-repair and adaptor ligation with NEB modules, biotin labeled validated chimeric DNA were selected with Streptavidin C1 beads (Invitrogen). Hi-C library was amplified after DNA elution by inculating at 98° C. Hi-C GENOMIC other PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
Release date2020-05-02
Run accession Release date Run data file information
File nameFile size (MB)
CRR133862 2020-05-02 hic_rep2_3d_1_R1.fastq.gz
SubmitterHUA LI (kaikaixinxin@sjtu.edu.cn)
OrganizationShanghai Jiao Tong University
Date submitted2020-04-30
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