| 实验编号 | CRX109901 |
| 物种名称 | Severe acute respiratory syndrome coronavirus 2 |
| 标题 | DTcov_0311_14_Sherry_enrich3 |
| 项目编号 | PRJCA002533 |
| 样本编号 | SAMC184986 |
| 测序平台 | Illumina Nextseq 500 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
We performed nucleic acid extraction, follwed by rRNA removal and DNA digestion. We then performed SHERRY-based sequencing library construction with 200 pmole random decamer supplemented during reverse transcription. For SARS-CoV15 sequence enrichment, we conducted in-solution hybridization with biotinylated RNA probes targeting the whole viral genome |
RNA-Seq |
METATRANSCRIPTOMIC |
Hybrid Selection |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 77
Planned read length (bp) for mate 2: 77
Insert size (bp): 350
|
| 发布日期 | 2022-04-30 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR135350 |
CRR135350.bam
|
7.94
|
|
| 提交者 | Hui Zeng (zenghui@ccmu.edu.cn) |
|---|
| 所属单位 | Beijing Ditan Hospital, Capital Medical University |
|---|
| 提交日期 | 2020-05-09 |
|---|
