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实验基本信息
实验编号 CRX113826
物种名称 Mus musculus
标题 RNA-seq of mouse brain I5
项目编号 PRJCA002742
样本编号 SAMC187867
测序平台 Illumina HiSeq X Ten
建库信息
文库名称 文库构建方法 建库策略 样品来源 实验选择 文库布局
A total amount of 3 g RNA of ischemic area in MCAO mouse brain was used as input material for rRNA removal using Epicentre Ribo-Zero Gold Kit (Epicentre, USA). Library construction and sequencing were performed by Annoroad Gene Technology (Beijing, China). Sequencing libraries were generated using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturers recommendations and index codes were added to attribute sequences to each sample. RNA-Seq TRANSCRIPTOMIC RANDOM PAIRED
处理信息 Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 350
发布日期2020-06-01
测序反应
Run编号 Run序列文件信息
File nameFile size (MB)
CRR139410 CRR139410_f1.fq.gz
CRR139410_r2.fq.gz
3,284.69
3,483.26
提交者Zelin Liu (zlliu@bjmu.edu.cn)
所属单位Peking University Health Science Center
提交日期2020-05-27
关联实验
Experiments(5)