| 实验编号 | CRX117288 |
| 物种名称 | Scylla paramamosain |
| 标题 | Sequencing the transcriptome of copulatory organ from adult male crabs. |
| 项目编号 | PRJCA001790 |
| 样本编号 | SAMC191306 |
| 测序平台 | Illumina HiSeq 3000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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RNA-seq transcriptome library was prepared following TruSeqTM RNA sample preparation Kit from Illumina using 5μg of total RNA. Shortly, messenger RNA was isolated according to poly A selection method by oligo dT beads and then fragmented, 100bp to 400bp, by fragmentation buffer firstly. Secondly double-stranded cDNA was synthesized using a SuperScript double-stranded cDNA synthesis kit with random hexamer primers. Then the synthesized cDNA was subjected to end-repair, phosphorylation and 'A' base addition according to Illumina's library construction protocol. Libraries were size selected for cDNA target fragments of 200-300 bp on 2% Low Range Ultra Agarose followed by PCR amplified using Phusion DNA polymerase for 15 PCR cycles. After quantified by TBS380, paired-end RNA-seq sequencing library was sequenced with the Illumina HiSeq 3000, 2 * 150bp read length. |
RNA-Seq |
TRANSCRIPTOMIC |
RT-PCR |
PAIRED
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| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 250
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| 发布日期 | 2021-12-01 |
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| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
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| CRR143135 |
CRR143135_f1.fq.gz
CRR143135_r2.fq.gz
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1,260.37
1,445.22
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| 提交者 | Ming Zhao (mingzhao1@hotmail.com) |
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| 所属单位 | East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences |
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| 提交日期 | 2020-06-04 |
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