Experiment information
Accession CRX128213
Organism Solanum lycopersicum
Title SGJ_1
BioProject PRJCA003184
BioSample SAMC204500
Platform Illumina NovaSeq 5000
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA was isolated from samples using the Trizol reagent (Invitrogen, USA) according to the manufacturer’s instructions. RNA quality and quantity were determined with a spectrophotometer (IMPLEN, Germany) and an Agilent 2100 Bioanalyzer (Agilent Technologies, USA). RNA-seq was performed at the Novogene Company (Beijing, China). The RNA-Seq library was constructed using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA). The mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. The cleaved RNA fragments were transcribed into first-strand cDNA using reverse transcriptase and subsequently second-strand cDNA synthesis was performed using DNA polymerase I and RNase H. The fragments were ligated to sequencing adaptors and the library preparations were sequenced on an Illumina HiSeq 4000 platform and 150 bp paired-end reads were generated. RNA-Seq TRANSCRIPTOMIC PolyA PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 350
Release date2022-08-05
Run
Run accession Run data file information
File nameFile size (MB)
CRR154393 CRR154393_f1.fq.gz
CRR154393_r2.fq.gz
1,414.27
1,552.15
SubmitterGuojing Shen (shenguojing@mail.kib.ac.cn)
OrganizationChinese Academy of Sciences
Date submitted2020-08-05