| 实验编号 | CRX130372 |
| 物种名称 | Solanum lycopersicum |
| 标题 | SGJ_6 |
| 项目编号 | PRJCA003220 |
| 样本编号 | SAMC206741 |
| 测序平台 | Illumina NovaSeq 5000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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Total RNA was isolated from samples using the Trizol reagent (Invitrogen, USA) according to the manufacturer’s instructions. RNA quality and quantity were determined with a spectrophotometer (IMPLEN, Germany) and an Agilent 2100 Bioanalyzer (Agilent Technologies, USA). RNA-seq was performed at the Novogene Company (Beijing, China). The RNA-Seq library was constructed using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA). The mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. The cleaved RNA fragments were transcribed into first-strand cDNA using reverse transcriptase and subsequently second-strand cDNA synthesis was performed using DNA polymerase I and RNase H. The fragments were ligated to sequencing adaptors and the library preparations were sequenced on an Illumina HiSeq 4000 platform and 150 bp paired-end reads were generated. |
RNA-Seq |
TRANSCRIPTOMIC |
PolyA |
PAIRED
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| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 350
|
| 发布日期 | 2022-08-10 |
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| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR156557 |
CRR156557_f1.fq.gz
CRR156557_r2.fq.gz
|
1,691.26
1,811.74
|
|
| 提交者 | Guojing Shen (shenguojing@mail.kib.ac.cn) |
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| 所属单位 | Chinese Academy of Sciences |
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| 提交日期 | 2020-08-10 |
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