Experiment information
Accession CRX149739
Organism Severe acute respiratory syndrome coronavirus 2
Title Genome sequencing of SARS-CoV-2 isolates from E86 by Probe
BioProject PRJCA003179
BioSample SAMC204307
Platform Illumina HiSeq X Ten
Library name Construction protocol Strategy Source Selection Layout
Inactivated total RNA was reverse transcribed into the first strand cDNA and the second strand cDNA was also synthesized. The cDNA was amplified with SPIA and library was prepared and ribosomal cDNA was depleted using Nugen Trio RNA-seq kit according to the manufacter's protocol. The SARS-COV-2 target library was enriched with iGeneTech TargetSeq One nCov kit. The prepared library was sequenced on Ilumina HiSeq X 10 with pair-end 2*150bp read length. RNA-Seq VIRAL RNA Hybrid Selection PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2021-06-07
Run accession Release date Run data file information
File nameFile size (MB)
CRR175948 2021-06-07 E65-96-E86_FKDL202580235-1A-GF03_1.fq.gz
Submitterli cp (licp@big.ac.cn)
OrganizationBeijing Institute of Genomics, Chinese Academy of Sciences
Date submitted2020-08-21
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