| Accession | CRX149753 |
| Organism | Severe acute respiratory syndrome coronavirus 2 |
| Title | Genome sequencing of SARS-CoV-2 isolates from E100 by Probe |
| BioProject | PRJCA003179 |
| BioSample | SAMC204321 |
| Platform | Illumina HiSeq X Ten |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Inactivated total RNA was reverse transcribed into the first strand cDNA and the second strand cDNA was also synthesized. The cDNA was amplified with SPIA and library was prepared and ribosomal cDNA was depleted using Nugen Trio RNA-seq kit according to the manufacter's protocol. The SARS-COV-2 target library was enriched with iGeneTech TargetSeq One nCov kit. The prepared library was sequenced on Ilumina HiSeq X 10 with pair-end 2*150bp read length. |
RNA-Seq |
VIRAL RNA |
Hybrid Selection |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2021-06-07 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR175962 |
CRR175962_f1.fq.gz
CRR175962_r2.fq.gz
|
265.57
291
|
|
| Submitter | li cp (licp@big.ac.cn) |
|---|
| Organization | Beijing Institute of Genomics, Chinese Academy of Sciences |
|---|
| Date submitted | 2020-08-21 |
|---|
