| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Genomic DNA was extracted using the QIAGEN Genomic DNA extraction kit following the manufacturer’s protocol. The extracted DNA was detected by NanoDropTM One UV-Vis spectrophotometer (Thermo Fisher Scientific, USA) for DNA purity, then Qubit 3.0 Fluorometer (Invitrogen, USA) was used to quantify DNA accurately. Approximately 15 μg of genomic DNA was size-selected using the BluePippin system (Sage Science, USA), and processed according to the protocol of Ligation Sequencing Kit (SQK-LSK109). The final library was sequenced on a PromethION sequencer (Oxford Nanopore Technologies, UK) with a running time of 48 hours. |
WGS |
GENOMIC |
DNAse |
SINGLE
|
|
