| Accession | CRX150697 |
| Organism | Populus alba |
| Title | PalF_nano |
| BioProject | PRJCA002485 |
| BioSample | SAMC153573 |
| Platform | OXFORD_NANOPORE PromethION |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Genomic DNA was extracted using the QIAGEN Genomic DNA extraction kit following the manufacturer’s protocol. The extracted DNA was detected by NanoDropTM One UV-Vis spectrophotometer (Thermo Fisher Scientific, USA) for DNA purity, then Qubit 3.0 Fluorometer (Invitrogen, USA) was used to quantify DNA accurately. Approximately 15 μg of genomic DNA was size-selected using the BluePippin system (Sage Science, USA), and processed according to the protocol of Ligation Sequencing Kit (SQK-LSK109). The final library was sequenced on a PromethION sequencer (Oxford Nanopore Technologies, UK) with a running time of 48 hours. |
WGS |
GENOMIC |
DNAse |
SINGLE
|
|
| Processing |
Planned read length (bp): 45000
|
| Release date | 2020-12-05 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR176925 |
CRR176925.fq.gz
|
5,738.99
|
| CRR176926 |
CRR176926.fq.gz
|
3,708.09
|
| CRR176927 |
CRR176927.fq.gz
|
4,720.72
|
| CRR176928 |
CRR176928.fq.gz
|
5,402.52
|
| CRR176929 |
CRR176929.fq.gz
|
5,548.39
|
|
| Submitter | Wenlu Yang (1123875647@qq.com) |
|---|
| Organization | Sichuan University |
|---|
| Date submitted | 2020-08-26 |
|---|
