| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Ethanol precipitation protocol and CTAB-PBIOZOL reagent was used for the purification of total RNA from the flower buds according to the manual instructions. the small RNA regions corresponding to the 18-30 nt bands in the marker lane (14-30 ssRNA Ladder Marker, TAKARA) were excised and recovered. The small RNA libraries were then produced using standardized procedures including adapter ligation, cDNA synthesis and size selection. The prepared library was sequenced using the DNBSEQ platform (BGI- Shenzhen, China) |
miRNA-Seq |
TRANSCRIPTOMIC |
cDNA |
SINGLE
|
|
