Accession | CRX168707 |
Organism | Sorghum bicolor |
Title | H3K36me3 |
BioProject | PRJCA003748 |
BioSample | SAMC254747 |
Platform | Illumina NovaSeq 6000 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The ChIP experiment was performed as described (Chen et al., 2013). About 2 g of 12-day-old of seedling leaves was cross-linked in 1% formaldehyde under vacuum. Chromatin was extracted and fragmented to 200-500 bp by sonication, and ChIP was performed using the following antibodies: anti-H3K36me3 (ab9050). DNA from ChIP was used to construct sequencing libraries following the protocol provided by Illumina TruSeq® ChIP Sample Prep Set A. The library construction and sequencing were completed at Novogene (Tianjin,China), and the library products were sequenced with the Illumina HiSeq platform. |
ChIP-Seq |
GENOMIC |
RANDOM PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
Release date | 2021-06-10 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR196813 |
CRR196813_f1.fq.gz
CRR196813_r2.fq.gz
|
1,423.43
1,494.04
|
|
Submitter | Chao Zhou (zhouchao@ctgu.edu.cn) |
Organization | China Three Gorges University |
Date submitted | 2020-10-27 |