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RNA were extracted from infected cells using TRIzol reagent (Invitrogen) and were further enriched for mRNA using Dynabeads mRNA Purification Kit (Invitrogen, Cat. No. 61006), followed by fragmentation, reverse transcription, and double-stranded cDNA synthesis. We ligated adaptor at the end of the repaired double-stranded cDNA and cyclized the PCR products using MGIEasy mRNA Library Prep Kit (no. 85-05536-01, MGI Technology, Shenzhen, China), the libraries were loaded into the chip of the MGI2000 platform for paired-ends sequencing based on DNA nano ball (DNB) technology, with a read length of 150 bp. |
RNA-Seq |
TRANSCRIPTOMIC |
PolyA |
PAIRED
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