| Accession | CRX197321 |
| Organism | Mus musculus |
| Title | E14.5 Embryo S4 |
| BioProject | PRJCA002426 |
| BioSample | SAMC297203 |
| Platform | Illumina NovaSeq 6000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
mouse E14.5 embryos were embedded in tissue-Tek (OCT) and snap-frozen using dry ice slurry. Then, to enable spatial gene analyses, the embryos were crysectioned at 10 um thickness and patial transcriptomics slides were printed with four identical 6.5x6.5 mm capture areas. The optimized permeabilization treatment times were identified as 20 mins for selected four slices. |
RNA-Seq |
TRANSCRIPTOMIC |
PolyA |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2021-07-21 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR230168 |
CRR230168_f1.fq.gz
CRR230168_r2.fq.gz
|
35,247.97
30,732.45
|
|
| Submitter | Suwei Gao (gaosuwei11@163.com) |
|---|
| Organization | Institute of Zoology, Chinese Academy of Sciences |
|---|
| Date submitted | 2020-12-22 |
|---|
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