| Accession | CRX197394 |
| Organism | Arabidopsis thaliana |
| Title | protoplasting free nuclei endosperm ngs |
| BioProject | PRJCA004059 |
| BioSample | SAMC297238 |
| Platform | Illumina NovaSeq 5000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
nuclei were loaded and subjected to library construction. To obtain full-length cDNA, we extend the elongation time during cDNA amplification from the standard 1 min to 2 minutes. Half of the cDNA template was used to construct Illumina library according to the manufacturer’s instruction and sequenced with Illumina NavoSeq (Read1:28 bases + Read2:150 bases) |
RNA-Seq |
TRANSCRIPTOMIC SINGLE CELL |
PolyA |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 28
Planned read length (bp) for mate 2: 150
|
| Release date | 2022-01-01 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR230241 |
CRR230241_f1.fastq.gz
CRR230241_r2.fastq.gz
|
44,814.18
45,953.47
|
|
| Submitter | Zhijian Liu (11930685@mail.sustech.edu.cn) |
|---|
| Organization | Southern University of Science and Technology |
|---|
| Date submitted | 2020-12-22 |
|---|
