| 实验编号 | CRX202254 |
| 物种名称 | Papaver rhoeas |
| 标题 | Oxford Nanopore sequencing raw data for Papaver rhoeas |
| 项目编号 | PRJCA004217 |
| 样本编号 | SAMC307749 |
| 测序平台 | OXFORD_NANOPORE PromethION |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
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For regular Oxford Nanopore (ONT) sequencing, 4 ug HMW DNA was used as input material for the ONT library preparations. Size-select of long DNA fragments for qualified samples were performed using the PippinHT system (Sage Science, USA). Next, the ends of DNA fragments were repaired, and A-ligation reaction was performed with NEBNext Ultra II End Repair/dA-tailing Kit (New England Biolabs Cat# E7546). The adapter in the SQK-LSK109 (Oxford Nanopore Technologies, UK) was used for further ligation reaction and DNA liabrary was measured by Qubit 4.0 Fluorometer (Invitrogen, USA). For ONT ultral-long sequencing, approximately 10 ug of ultra-long gDNA was size selected (>50 kb) with SageHLS HMW library system (Sage Science, USA), and processed using the Ligation sequencing 1D kit (SQK-LSK109, Oxford Nanopore Technologies, UK) according the manufacturer's instructions. |
WGS |
GENOMIC |
unspecified |
SINGLE
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| 处理信息 |
Planned read length (bp): 30000
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| 发布日期 | 2021-03-23 |
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| 测序反应 |
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| 提交者 | Xiaofei Yang (xfyang@xjtu.edu.cn) |
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| 所属单位 | Xi'an Jiaotong University |
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| 提交日期 | 2021-01-10 |
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