| 实验编号 | CRX202262 |
| 物种名称 | Papaver rhoeas |
| 外部数据库编号 |
: |
| 标题 | Leaf Hi-C sequencing raw data for Papaver rhoeas |
| 项目编号 | PRJCA004217 |
| 样本编号 | SAMC307749 |
| 测序平台 | Illumina NovaSeq 5000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
Chromatin was digested for 16 h with 400 U HindIII restriction enzyme (NEB) at 37 centigrade. DNA ends were labeled with biotin and incubated at 37 centigrade for 45 min, and the enzyme was inactivated with 20% SDS solution. DNA ligation was performed by the addition of T4 DNA ligase (NEB) and incubation at 16 centigrade for 4~6 h. After ligation, proteinase K was added to reverse cross-linking during incubation at 65 centigrade overnight. DNA fragments were purified and dissolved in 86 mL of water. Unligated ends were then removed. Purified DNA was fragmented to a size of 300-500 bp, and DNA ends were then repaired. DNA fragments labeled by biotin were finally separated on Dynabeads M-280 Streptavidin (Life Technologies). |
Hi-C |
GENOMIC |
Restriction Digest |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| 发布日期 | 2021-03-23 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR240490 |
CRR240490_f1.fastq.gz
CRR240490_r2.fastq.gz
|
7,624.94
8,008.57
|
| CRR240491 |
CRR240491_f1.fastq.gz
CRR240491_r2.fastq.gz
|
7,863.94
8,161.42
|
| CRR240492 |
CRR240492_f1.fastq.gz
CRR240492_r2.fastq.gz
|
46,496.43
48,055.35
|
| CRR240493 |
CRR240493_f1.fastq.gz
CRR240493_r2.fastq.gz
|
15,887.62
17,300.05
|
| CRR240494 |
CRR240494_f1.fastq.gz
CRR240494_r2.fastq.gz
|
7,329.78
7,598.46
|
|
| 提交者 | Xiaofei Yang (xfyang@xjtu.edu.cn) |
|---|
| 所属单位 | Xi'an Jiaotong University |
|---|
| 提交日期 | 2021-01-10 |
|---|
