Accession | CRX204653 |
Organism | Mus musculus |
Title | NM_RA_m11to15_rep1 |
BioProject | PRJCA003611 |
BioSample | SAMC311599 |
Platform | Illumina HiSeq 4000 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
NM_RA_m11to15_rep1 |
The heart tissues were thoroughly ground at low temperatures. The tissue pellets were resuspended in 500 µl ice-cold lysis buffer with Protease Inhibitor Cocktail: 10 mM Tris·HCl (pH 7.5), 10 mM NaCl, 3 mM MgCl2, 0.1 mM EDTA, and 0.5% NP-40. The tubes were incubated for 60 min with vortexing every 10 min to release nuclei. |
OTHER |
GENOMIC |
size fractionation |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
|
Release date | 2021-03-29 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR243374 |
CRR243374_f1.fastq.gz
CRR243374_r2.fastq.gz
|
6,576.24
7,075.6
|
|
Submitter | Fuchou Tang (tangfuchou@pku.edu.cn) |
Organization | Peking University |
Date submitted | 2021-01-21 |