| Accession | CRX204664 |
| Organism | Mus musculus |
| Title | RM_LV_m1to5 |
| BioProject | PRJCA003611 |
| BioSample | SAMC311610 |
| Platform | Illumina HiSeq 4000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
| RM_LV_m1to5 |
The heart tissues were thoroughly ground at low temperatures. The tissue pellets were resuspended in 500 µl ice-cold lysis buffer with Protease Inhibitor Cocktail: 10 mM Tris·HCl (pH 7.5), 10 mM NaCl, 3 mM MgCl2, 0.1 mM EDTA, and 0.5% NP-40. The tubes were incubated for 60 min with vortexing every 10 min to release nuclei. |
RNA-Seq |
TRANSCRIPTOMIC |
size fractionation |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
|
| Release date | 2021-03-29 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR243385 |
CRR243385_f1.fastq.gz
CRR243385_r2.fastq.gz
|
1,100.38
1,174.54
|
|
| Submitter | Fuchou Tang (tangfuchou@pku.edu.cn) |
|---|
| Organization | Peking University |
|---|
| Date submitted | 2021-01-21 |
|---|
