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Experiment information
Accession CRX204665
Organism Mus musculus
Title RM_LV_m6to10
BioProject PRJCA003611
BioSample SAMC311611
Platform Illumina HiSeq 4000
Library
Library name Construction protocol Strategy Source Selection Layout
RM_LV_m6to10 The heart tissues were thoroughly ground at low temperatures. The tissue pellets were resuspended in 500 µl ice-cold lysis buffer with Protease Inhibitor Cocktail: 10 mM Tris·HCl (pH 7.5), 10 mM NaCl, 3 mM MgCl2, 0.1 mM EDTA, and 0.5% NP-40. The tubes were incubated for 60 min with vortexing every 10 min to release nuclei. RNA-Seq TRANSCRIPTOMIC size fractionation PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
Release date2021-03-29
Run
Run accession Run data file information
File nameFile size (MB)
CRR243386 CRR243386_f1.fastq.gz
CRR243386_r2.fastq.gz 		987.29
1,061.86
SubmitterFuchou Tang (tangfuchou@pku.edu.cn)
OrganizationPeking University
Date submitted2021-01-21
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