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Experiment information

Accession

CRX204667

Organism

Title

RM_RA_m1to5

BioProject

BioSample

Platform

Illumina HiSeq 4000

Library

Library name	Construction protocol	Strategy	Source	Selection	Layout
RM_RA_m1to5	The heart tissues were thoroughly ground at low temperatures. The tissue pellets were resuspended in 500 µl ice-cold lysis buffer with Protease Inhibitor Cocktail: 10 mM Tris·HCl (pH 7.5), 10 mM NaCl, 3 mM MgCl2, 0.1 mM EDTA, and 0.5% NP-40. The tubes were incubated for 60 min with vortexing every 10 min to release nuclei.	RNA-Seq	TRANSCRIPTOMIC	size fractionation	PAIRED

Processing

Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300

Release date

2021-03-29

Run

Run accession	Run data file information
Run accession	File name	File size (MB)
CRR243388	CRR243388_f1.fastq.gz CRR243388_r2.fastq.gz	972.08 1,035.57

Submitter

Fuchou Tang (tangfuchou@pku.edu.cn)

Organization

Peking University

Date submitted

2021-01-21

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