The single cell RNA libraries were prepared using the ChromiumTM Single cell 5’ Reagent Kit of Chromium platform (10x Genomics, USA) following the manufacturer’s instruction. Generated single cell RNA libraries were sequenced on the Illumina HiSeq X Ten platform. The CellRanger software (version 3.1.0) was used for preprocessing of the PE150 Illumina sequencing reads.
RNA-Seq
TRANSCRIPTOMIC
RANDOM
PAIRED
Processing
Planned read length (bp) for mate 1: 150 Planned read length (bp) for mate 2: 150