Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The extracted genomic DNA was qualified, size-selected using the BluePippin system (Sage Science). Then, the genomic DNA was end-repaired and PCR adapters supplied in ONT sequencing kit (SQK-LSK109) were ligated to the end-repaired DNA. Finally, Qubit® 3.0 Fluorometer (Invitrogen) was used to quantify the size of library fragments. To generate Nanopore reads, the prepared libraries are loaded into flow cells (R9.4, FLO-PRO002) of a PromethION sequencer (ONT). |
WGS |
GENOMIC |
unspecified |
SINGLE
|
|