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Experiment information
Accession CRX220377
Organism Oryza sativa Japonica Group
Title rice_rep2_nanopore
BioProject PRJCA004326
BioSample SAMC316303
Platform OXFORD_NANOPORE PromethION
Library
Library name Construction protocol Strategy Source Selection Layout
The extracted genomic DNA was qualified, size-selected using the BluePippin system (Sage Science). Then, the genomic DNA was end-repaired and PCR adapters supplied in ONT sequencing kit (SQK-LSK109) were ligated to the end-repaired DNA. Finally, Qubit® 3.0 Fluorometer (Invitrogen) was used to quantify the size of library fragments. To generate Nanopore reads, the prepared libraries are loaded into flow cells (R9.4, FLO-PRO002) of a PromethION sequencer (ONT). WGS GENOMIC unspecified SINGLE
Processing Planned read length (bp): 23790
Release date2021-02-09
Run
Run accession Run data file information
File nameFile size (MB)
CRR261445 CRR261445.tar.gz 561,094.7
SubmitterPeng Ni (nipeng@csu.edu.cn)
OrganizationCentral South University
Date submitted2021-02-05
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