| 实验编号 | CRX223965 |
| 物种名称 | soil metagenome |
| 标题 | F-TP4-1 |
| 项目编号 | PRJCA001644 |
| 样本编号 | SAMC338332 |
| 测序平台 | Illumina HiSeq 2500 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
Total fungi genomic DNA samples were extracted using DNA extraction kit for the corresponding sample. The concentration and purity were measured using the NanoDrop One. The ITS2 region of 16S rRNA in fungi was amplified by PCR with forward primer ITS3F and reverse primer ITS4R. Sample-specific 12-bp barcode sequences was added to the same DNA library to distinguish different samples. Primers were synthesized by Invitrogen. At last, the library was sequenced on an IlluminaHiseq2500 platform and 250 bp paired-end reads were generated. |
AMPLICON |
METAGENOMIC |
PCR |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 450
Planned read length (bp) for mate 2: 450
|
| 发布日期 | 2021-02-10 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR266064 |
CRR266064_f1.fq.gz
CRR266064_r2.fq.gz
|
5.71
6.63
|
|
| 提交者 | Dianming Wu (dmwu@geo.ecnu.edu.cn) |
|---|
| 所属单位 | East China Normal University |
|---|
| 提交日期 | 2021-02-10 |
|---|
