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RNA was harvested using Trizol reagent.RNA quality and quantity was measured by using nanodrop spectrophotometer (ND-1004, Nanodrop Technologies) and RNA Integrity was determined by gel electrophoresis,Basecalls performed using Solexa pipeline,The trimmed reads (trimmed 5',3'-adaptor bases using cutadapt) were aligned to reference genome using Hisat2 software,The transcript abundances for each sample was estimated with StringTie,the FPKM value for gene and transcript level were calculated with R package Ballgown,The circRNA reads were aligned to reference genome using STAR detecting backesplicec junction reads with CIRCexplore2 |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
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