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Experiment information
Accession CRX227974
Organism Mus sp.
Title N2
BioProject PRJCA004520
BioSample SAMC339999
Platform Illumina NovaSeq 6000
Library
Library name Construction protocol Strategy Source Selection Layout
RNA was harvested using Trizol reagent.RNA quality and quantity was measured by using nanodrop spectrophotometer (ND-1004, Nanodrop Technologies) and RNA Integrity was determined by gel electrophoresis,Basecalls performed using Solexa pipeline,The trimmed reads (trimmed 5',3'-adaptor bases using cutadapt) were aligned to reference genome using Hisat2 software,The transcript abundances for each sample was estimated with StringTie,the FPKM value for gene and transcript level were calculated with R package Ballgown,The circRNA reads were aligned to reference genome using STAR detecting backesplicec junction reads with CIRCexplore2 RNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2021-08-22
Run
Run accession Run data file information
File nameFile size (MB)
CRR270268 CRR270268_f1.fastq.gz
CRR270268_r2.fastq.gz
2,493.21
2,580.54
SubmitterJian HE (hejian2011@126.com)
Organizationthe first people's hospital of foshan
Date submitted2021-02-23
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Experiments(5)