| 实验编号 | CRX237337 |
| 物种名称 | Crassostrea hongkongensis |
| 标题 | Hongkong oyster5 |
| 项目编号 | PRJCA004719 |
| 样本编号 | SAMC352488 |
| 测序平台 | PacBio RS II |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
Genomic DNA was sheared by a g-TUBE device with 20 kb settings. Sheared DNA was then purified and concentrated with AMPure XP beads and further used for single-molecule real-time (SMRT) bell preparation according to the manufacturer’s protocol , and 20 kb template preparation by using BluePippin size selection (Sage Science). Size selected and isolated SMRT bell fractions were purified with AMPure XP beads. Finally, these purified SMRT bells were used for primer and polymerase (P6) binding, according to manufacturer’s binding calculator (Pacific Biosciences). Single-molecule sequencing was performed on a PacBio RS-II platform with C4 chemistry. Only PacBio subreads no shorter than 500 bp were included for performing oyster genome assembly. |
WGS |
GENOMIC |
RANDOM |
SINGLE
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| 发布日期 | 2021-05-31 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR280606 |
CRR280606.fastq.gz
|
98.87
|
| CRR280607 |
CRR280607.fastq.gz
|
146.62
|
| CRR280608 |
CRR280608.fastq.gz
|
153.59
|
|
| 提交者 | Yang Zhang (yzhang@scsio.ac.cn) |
|---|
| 所属单位 | South China Sea Institute of Oceanology, Chinese Academy of Sciences |
|---|
| 提交日期 | 2021-04-29 |
|---|
