Experiment information
Accession CRX238874
Organism Mus musculus
Title WT-D18-6
BioProject PRJCA004965
BioSample SAMC354638
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
For tumors, collected tumors were cut large into samples with size less than 0.5 cm in dimension and then the fresh tissues were placed in 10 volumes of RNAlater Solution.RNA isolation, library construction and sequencing were performed by WuXi NextCODE. RNeasy MinElute Cleanup Kit (QIAGEN, 74204) was used for RNA extraction, followed by generating mRNA-focused sequencing libraries from total RNA using Illumina TruSeq RNA Sample Preparation Kit v2. Paired-end 150 bp sequencing was done on illumina Hiseq X10 (40M reads for each sample). RNA-Seq TRANSCRIPTOMIC PolyA PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2021-09-15
Run
Run accession Run data file information
File nameFile size (MB)
CRR282225 CRR282225_f1.fastq.gz
CRR282225_r2.fastq.gz
4,259.3
4,849.16
SubmitterTian-Yu Song (songtianyu@zju.edu.cn)
OrganizationShanghaiTech University
Date submitted2021-05-08