Accession | CRX238876 |
Organism | Mus musculus |
Title | WT-D18-8 |
BioProject | PRJCA004965 |
BioSample | SAMC354640 |
Platform | Illumina HiSeq X Ten |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
For tumors, collected tumors were cut large into samples with size less than 0.5 cm in dimension and then the fresh tissues were placed in 10 volumes of RNAlater Solution.RNA isolation, library construction and sequencing were performed by WuXi NextCODE. RNeasy MinElute Cleanup Kit (QIAGEN, 74204) was used for RNA extraction, followed by generating mRNA-focused sequencing libraries from total RNA using Illumina TruSeq RNA Sample Preparation Kit v2. Paired-end 150 bp sequencing was done on illumina Hiseq X10 (40M reads for each sample). |
RNA-Seq |
TRANSCRIPTOMIC |
PolyA |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
Release date | 2021-09-15 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR282227 |
CRR282227_f1.fastq.gz
CRR282227_r2.fastq.gz
|
3,777.24
4,311.2
|
|
Submitter | Tian-Yu Song (songtianyu@zju.edu.cn) |
Organization | ShanghaiTech University |
Date submitted | 2021-05-08 |