| 实验编号 | CRX238886 |
| 物种名称 | Mus musculus |
| 标题 | aPD1-5 |
| 项目编号 | PRJCA004965 |
| 样本编号 | SAMC354650 |
| 测序平台 | Illumina HiSeq X Ten |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
For tumors, collected tumors were cut large into samples with size less than 0.5 cm in dimension and then the fresh tissues were placed in 10 volumes of RNAlater Solution.RNA isolation, library construction and sequencing were performed by WuXi NextCODE. RNeasy MinElute Cleanup Kit (QIAGEN, 74204) was used for RNA extraction, followed by generating mRNA-focused sequencing libraries from total RNA using Illumina TruSeq RNA Sample Preparation Kit v2. Paired-end 150 bp sequencing was done on illumina Hiseq X10 (40M reads for each sample). |
RNA-Seq |
TRANSCRIPTOMIC |
PolyA |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| 发布日期 | 2021-09-15 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR282237 |
CRR282237_f1.fastq.gz
CRR282237_r2.fastq.gz
|
4,585.88
5,173.22
|
|
| 提交者 | Tian-Yu Song (songtianyu@zju.edu.cn) |
|---|
| 所属单位 | ShanghaiTech University |
|---|
| 提交日期 | 2021-05-08 |
|---|
