Experiment information
Accession CRX238930
Organism Mus musculus
Title No T-Day2.5_1
BioProject PRJCA004965
BioSample SAMC354669
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
OT-1 T cells were isolated from spleen and lymph nodes of OT-1 transgenic mouse and stimulated with recombinant mouse IL-2, mouse IL-7 and mouse IL-15 for two days. For T cell co-culture screen, MC38-OVA cells transduced with mMCE sgRNA library post puromycin selection were seeded with library coverage more than 2000 fold in each T750 flask (total 12 flasks). Next day, effector T cells were planted in each flask with mouse IL-2 added. MC38-OVA cells with library coverage more than 4000 fold were collected as baseline. With no-T cells as the negative control, tumor cells were collected at day 1, day 2 and day 2.5 with library coverage more than 1000 fold post co-culture for NGS as described in the in vivo screen. AMPLICON OTHER PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2021-09-15
Run
Run accession Run data file information
File nameFile size (MB)
CRR282281 CRR282281_f1.fastq.gz
CRR282281_r2.fastq.gz
958.31
976.02
SubmitterTian-Yu Song (songtianyu@zju.edu.cn)
OrganizationShanghaiTech University
Date submitted2021-05-09